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Figure 3 590315

Figure 3 590315
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Particle Sizing

Assessing particle size distributions correctly relies on a suite of techniques since no single technique can cover the full range. Small biological assemblies or nanoparticles (1 to 70-80 nm) are best determined through dynamic light scattering (A: bovine serum albumin in solution). Larger nanoparticles or polydisperse sub-micron colloids (50 to circa 700 nm) are best measured by Nanoparticle Tracking Analysis (B: size distribution of calcium phosphate colloids) as this technique relies upon direct visualisation of individual particles (C: calcium phosphate colloids). As we progress to larger sizes (200nm – 500 µm), sedimentation becomes a problem but can be addressed by coupling a dispersion unit to the particle sizer and performing Static Light Scattering analyses (D: large amorphous silica particles).
Particle Sizing
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