Control of influenza virus replication
My research focuses on three areas of virology:1. Transgenic strategies for controlling influenza virus replication in livestock hosts. We are exploring molecular approaches to interfere with influenza virus replication in vivo and testing these using transgenic livestock (chickens and pigs).
2. Enhancing vaccine production in eggs: By altering the expression pattern of viral receptors and disrupting antiviral pathways of host cells (specifically chick embryos in fertilised eggs) we are generating eggs that can propagate a wider range of viruses and produce higher yields for vaccine production.
3. Investigating the dynamics of influenza virus mixed infections in cell culture and in vivo. Using "barcoded" viruses we are investigating the frequency and consequences of mixed infections by influenza virus. We are particularly interested in how viral quasispecies can support the persistence of lower-fitness but evolutionarily significant variants within the population.
Asparagine 631 variants of the Chicken Mx protein do not inhibit influenza replication in primary chicken embryo fibroblast cells or in vitro surrogate assays. Benfield C., Lyall, J., Kochs, G. and Tiley, L.S. 2008. J. Virol 82:7533-7539
Suppression of avian influenza transmission in genetically modified chickens. Lyall. J.E., Irvine R.M., Sherman A., McKinley T.J., Núñez A., Purdie A., Outtrim L., Brown I., Rolleston-Smith G., Sang H. and Tiley L.S. 2011 Science. 331:223-226
Bourret, V., Lyall, J., Ducatez, M.F., Guérin, J.L. and Tiley, L. 2012. Development of an improved polykaryon-based influenza virus rescue system. BMC Biotechnol. 12:69.
The role and assembly mechanism of nucleoprotein in influenza A virus ribonucleoprotein complexes Turrell, L., Lyall, J.E., Tiley, L.S., Fodor, E. and Vreede, F.T. 2013. Nature Comms. 4:1591.
Whole-genome, deep pyrosequencing analysis of a duck influenza A virus evolution in swine cells. Bourret V, Croville G, Mariette J, Klopp C, Bouchez O, Tiley L, Guérin JL. 2013. Infect Genet Evol. 18:31-41. doi: 10.1016/j.meegid.2013.04.034. Epub 2013